Economou Lab-Teaching Microbial Protein Secretion and Biotechnology


Microorganisms as macromolecule factories


Graduate program  on Microbial Biotechnology, Dpt Biology, University of Athens

Model microbial systems for the study of genes of medical, agricultural and biotechnological importance
Lecture Lecturer Length Downloads

Proteins and protein machines

Economou 1h PDF QT
Protein folding in the cell: chaperones Economou 1h PDF QT
Protein export: Sec and bacterial pathogens Economou 3h PDF QT

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Recommended Textbooks:

1. Branden and Tooze, 1999 Introduction to Protein structure 2nd edition (home)

2. Petsko and Ringe, 2004 Protein structure and function (home)

3. Alberts et al, 2002 Molecular Biology of the Cell 4th edition (home)

4. Stryer, 2003 Biochemistry 5th edition (home); Greek translation 2004; Crete University Press (home)

5. Pollack, 2001 Cells and gels and the engines of life (home)

Further Reading:

(** essential)

Protein export: Sec and bacterial pathogens

**Pugsley AP, Francetic O, Driessen AJ, de Lorenzo V. (2004) Getting out: protein traffic in prokaryotes. Mol Microbiol. 52, 3-11.

Abstract PDF

Dalbey RE, Kuhn A. (2004) YidC family members are involved in the membrane insertion, lateral
integration, folding, and assembly of membrane proteins. J Cell Biol. 166, 769-774.

Abstract PDF

Randall LL, Hardy SJ. (2002) SecB, one small chaperone in the complex milieu of the cell. Cell Mol Life Sci. 59, 1617-1623.

Abstract PDF

Keenan RJ, Freymann DM, Stroud RM, Walter P. (2001) The signal recognition particle. Annu Rev Biochem.70:755-775

Abstract PDF

**Clemons WM Jr, Menetret JF, Akey CW, Rapoport TA. (2004) Structural insight into the protein translocation channel.
Curr Opin Struct Biol. 14, 390-396.

Abstract PDF

**Vrontou and Economou, A. (2004) Structure and function of SecA, the preprotein translocase nanomotor.BBA Mol Cell Reviews (in press)

Abstract PDF

*Gauthier A, Thomas NA, Finlay BB. (2003) Bacterial injection machines. J Biol Chem. 278, 25273-25276.

Abstract PDF

Protein folding in the cell: chaperones

Bukau B, Horwich AL.  (1998) The Hsp70 and Hsp60 chaperone machines.
Cell. 1998 Feb 6;92(3):351-66.

Abstract PDF

**Hartl FU, Hayer-Hartl M. (2002) Molecular chaperones in the cytosol: from nascent chain to folded protein.
Science. 295, 1852-1858.

Abstract PDF

Jason C. Young, Joseã M. Barral and F. Ulrich Hartl (2003) More than folding: localized functions
of cytosolic chaperones.TIBS 28, 541-547

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Choi JH, Lee SY. (2004) Secretory and extracellular production of recombinant proteins using Escherichia coli. Appl Microbiol Biotechnol. 64, :625-635.

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Steidler L, Hans W, Schotte L, Neirynck S, Obermeier F, Falk W, Fiers W, Remaut E. (2000) Treatment of murine colitis by Lactococcus lactis secreting interleukin-10. Science. 289, 1352-1355.

Abstract PDF

Dobson CM. (2002) Getting out of shape. Nature. 418, 729-730.

Abstract PDF